DENDRITIC CELLS ARE RESPONSIBLE FOR THE FAILURE IN CTLs GENERATION IN LSP1-DEFICIENT MICE

RACHEL PAOLA ACLAND STRACK, MARINE GROS, MERCEDES PASCUAL, BELKYS MALETTO, MARÍA CRISTINA PISTORESI, ANA MARÍA LENNON-DUMENIL, SEBASTIÁN AMIGORENA, GABRIEL MORÓN

Congreso; Reunión Sociedades de Biociencias; 2017

Leukocyte-specific protein 1 (LSP1) is an important regulator of actin cytoskeleton remodeling, modulating leukocytes motility, due to its F-actin binding domain. We have previously shown that Lsp1-/- mice have an impaired CTL response after antigen exposure. Moreover, Lsp1-/- dendritic cells (DCs) fail to induce a strong CTL response in vivo, migrate to lymphoid tissues, present antigens and produce IL-12 when transferred into WT mice. In order to deepen the mechanisms operating this diminished CTL generation in Lsp1-/- mice, we first analyzed the ability of their CD8+ T cells to proliferate and become activated. After in vitro stimulation with αCD3/CD28, Lsp1-/- CD8+ T cells proliferated and up-regulated CD25 as strongly as WT CD8+ T cells. Granzyme B production and IFNg release were slightly lower (p