NEUTROPHILS RECRUITMENT IMPACT ON T CELL RESPONSE IN LYMPH NODES.

CASTELL, SOFÍA D; HARMAN, MARÍA F; GORLINO, CAROLINA V; MORÓN, VICTOR G; MALETTO, BELKYS A; PISTORESI, MARÍA C

Mar del Plata

Congreso; LXII reunión de la Sociedad Argentina de Inmunología; 2014

Previously we demonstrated that when mice were immunized with OVA emulsified in Freund?s complete adjuvant (OVA/CFA) at days 0, 15, 30, and ten days after the last immunization were injected with OVA-FITC in the footpad, the main OVA-FITC+ cells recruited in draining popliteal lymph nodes were neutrophils, this influx was dependent on an antigen-specific response (Maletto et al, Blood, 2006; Gorlino et al, Journal of Immunology, 2014). In order to reduce CFA potentially harmful effects, in the present study we performed only two immunizations, for this, mice were immunized with OVA/CFA and 15 days after boosted with OVA/IFA; IFA is Freund?s adjuvant incomplete form that lacks the mycobacterial components. Ten days after the last immunization, mice were injected with OVA-FITC in the footpad and we still found neutrophils migration indraining popliteal lymph nodes (p