Synthesis and characterization of solid lipid nanoparticles for controlled release of Carbamazepine

SCIOLI MONTOTO, SEBASTIÁN; ISLAN, GERMAN; RUIZ, MARÍA ESPERANZA; CASTRO, GUILLERMO RAÚL

Trivandrum

Congreso; New Horizons in Biotechnology; 2015

CSIR-National Institute for Interdisciplinary Science and Technology

Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) have been shown to penetrate the Blood Brain Barrier and allow for greater delivery of drugs to the CNS. They present the same advantages of polymeric nanoparticles (NP) but with much lower intrinsic toxicity. The aim of the present work was the encapsulation of the antiepileptic drug Carbamazepine in SLN and NLC in order to develop a controlled release delivery system. As lipid phase, cetyl esters, myristyl myristate and Croda® GTCC-LQ-/SG oil were used. NP were prepared by ultrasonication of a mixture of the melted lipids and 3% solution of Kolliphor® P188 in water, previously heated at a temperature slightly above the lipids? mp. The drug was incorporated to the lipid phase, alone or dissolved in 50 l of DMSO. Formulations were observed by TEM and AFM, showing a mean size range of 50-500 nm. In vitro release studies were performed in 500 ml of KH2PO4 (pH 6.8) using Apparatus 2 USP (paddle) at 100 rpm. Four different formulations were assayed, and all of them showed high %EE (82.78-93.84%) with released percentages of (29.47-45.30%) and (50.80-78.53%) at 2 and 6 hours, respectively, reaching (70.34-99.59%) at 24 hours.