GENOME SEQUENCE ANALYSIS OF BRADYRHIZOBIUM YUANMINGENSE STRAIN P10 130, A HIGHLY EFFICIENT NITROGEN-FIXING BACTERIUM

VACCA, CAROLINA; CASTELLANI, LUCAS GABRIEL; CAFIERO, JUAN HILARIO; TONIUTTI, MARÍA ANTONIETA; ALBICORO, FRANCISCO JAVIER; LÓPEZ GARCÍA, SILVINA LAURA; FORNASERO, LAURA VIVIANA; ZUBER, NICOLÁS EMILIO; LAGARES, ANTONIO; TORRES TEJERIZO, GONZALO ARTURO; DEL PAPA, MARÍA FLORENCIA

Congreso; Reunion conjunta SAIB-SAMIGE 2020; 2020

Sociedad Argentina de Investigación Bioquímica y Biología Molecular y Sociedad Argentina de Microbiología General

The study of D. incanum-nodulating rhizobia is not only important for their role as microsymbionts of legumes with forage potential, but also for their role as a biotic factor for the establishment of Desmodium species in Argentinean soils. In a previous study Bradyrhizobium strain, P10 130, was selected as the best candidate for Desmodium incanum inoculation based on a broad selective criteria and growth plant promotion. MALDI BioTyper and 16S rRNA phylogenetic analysis let us determine a close relationship between this strain and B. yuanmingense. The study of taxonomy of Bradyrhizobium genus is still complex, but many modern studies indicate that data provided from genome sequence analysis may simplify taxonomic assignment. The objective of this study was to analyze the genome sequence of B. yuanmingense P10 130 to deepen our knowledge regarding its plant growth-promoting traits. Furthermore, genome analysis was used to establish its phylogenetic relationship with other species of Bradyrhizobium genus. We were able to estimate the genome size of strain P10 130 as 7.54 Mb that consisted of 65 contigs. We also performed Genome Average Nucleotide Identity (ANI) analysis, which revealed that B. yuanmingense CCBAU 10071T was the closest strain to P10 130 with ca. 96% identity. Moreover, analysis of the genome of B. yuanmingense P10 130 identified 20 nod/nol/noe, 14 nif/18 fix, 5 nap/5 nor genes, which may be potentially involved in nodulation, nitrogen fixation and denitrification, respectively. We present here the genome sequence of B. yuanmingense P10 130 as a valuable source of information to continue the research of its potential industrial production as a biofertilizer of D. incanum.