METFORMIN TREATMENT MODULATES MACROPHAGE RESPONSE AGAINST T. CRUZI INFECTION

RUTH ELIANA BAIGORRÍ; YAMILE ANA; BELEN BRUGO; VIANO, MARIA ESTEFANIA; RODRIGUEZ-GALAN, MARIA CECILIA; CRISTINA MOTRÁN; CINTHIA STEMPIN; CERBÁN, FABIO

Congreso; REUNION CONJUNTA SAIC-SAI-AAFE-NANOMED; 2021

In acute phase of T. cruzi infection, both innate and adaptive immunity are necessary tocontrol parasite replication. Macrophage (Mf) and T cells orchestrate the inflammatoryresponse that controls parasite burden. However, an exacerbated immune responseresults in tissue damage, mainly by ROS and RNS release. Metformin (Mf), a type 2diabetes drug, reduces inflammation in models of aging and pollution. In our in vivomodel of T. cruzi infection in Balb/c mice, we observed that peritoneal and spleen Mfincrease iNOS expression during acute phase. Pretreatment of BMDM with Mf preventsintracellular parasite replication and promotes proinflammatory cytokine production.We also infected RAW cells and then were treated with PBS or Mf 1mM. This treatmentdecreased ROS production (p