p300 involvement in apoptosis and metastasis in triple negative breast carcinoma

GUILLERMINA ANA GALLARDO; CLEMENTE VALENTINA; IBARRA AGUSTINA; FACCHINETTI MARIA MARTA; CURINO ALEJANDRO CARLOS; FERMENTO MARIA EUGENIA

Mar del Plata

Congreso; Reunion anula de sociedades de Biociencias; 2020

SAIC

Triple-negative breast cancer (TNBC) is a heterogeneous group of tumors that lack specific molecular targets. Therefore, it is necessary to investigate potential tumor markers for this subtype of BC. There are few studies in TNBC on the role of p300 in apoptosis and they are contradictory. Previously, we published that pharmacological inhibition of p300 increases apoptosis in murine BC cells, however, it is necessary to check whether the same effect occurs in human TNBC cells. Recently a prometastatic role of p300 in BC has been found. In addition, we demonstrated that pharmacological inhibition of p300 decreases migration and adhesion in TNBC, showing the need to delve into the molecules involved. Hence, the aim of this work was to study the effect of the inhibition of the acetylase function of p300 in the processes of apoptosis and metastasis in human TNBC cells. The MDA-MB-231 cell line was treated with VV59 (inhibitor of the acetylase function of p300) or its vehicle (DMSO). In a cell viability assay, we observed that the pharmacological inhibition of p300 produced a decrease in the number of cells compared to vehicle (p