Purinergic Signaling Controls T-Cell Response

BERGERO, GASTÓN; MAZZOCCO, YANINA; LIU, RUINING; DEL ROSSO, SEBASTIAN; ROTTENBERG, MARTIN; AOKI, MARIA DEL PILAR

San Luis

Congreso; Reunión Anual de la Sociedad Argentina de Inmunología; 2023

Sociedad Argentina de Inmunología

In response to hypoxic or inflammatory conditions, immune cells release ATP, which triggers multiple immune effector functions to fight infections. Its life span is determined by CD39 and CD73 purinergic ectoenzymes that coordinate ATP hydrolysis into the potent anti-inflammatory adenosine (ADO). The enzymatic activity of CD39 and CD73 could be boosted by HIF-1, leading to the generation of ADO, which in turn helps to reduce inflammation. How HIF-1 regulates ADO production by CD39 and CD73 and the impact on Tcell activation will be particularly addressed. We found that in vitro-activated CD4 T-cells increase the expression of ATP-receptor P2X7, CD39, CD73, and ADOreceptors A2a and A2b, and HIF-1α compared to naïve cells. CD73 activity inhibition (CD73KO), induced a higher activation state (CD44 and CD69) compared to WT cells (WT vs CD73KO: p