Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis : comparative study of fruit and leaves extracts

COGOI, LAURA; MARRASSINI, CARLA; SAINT MARTIN, ELINA MALEN; ALONSO, MARÍA ROSARIO; FILIP, ROSANA; ANESINI, CLAUDIA

Journal of Pharmacopuncture

Korean Pharmacopuncture Institute

Año: 2023 vol. 26 p. 338 - 347

2093-6966

Objectives: Ilex paraguariensis (Aquifoleaceae) is cultivated to produce “yerba mate”. Dueto its nutritional, energizing, hypoglycemic and antioxidant effects, it is used in the elaborationof food, pharmaceuticals, and cosmetics. The oxidative stress related to proteinglycation and production of advanced glycation end products (AGEs) leads to the developmentof several diseases. The objective of this work was to compare the antioxidant andanti-AGEs activity of a decoction of fruits (F) and leaves (L).Methods: The antioxidant activity was assayed by the DPPH assay and the inhibition ofegg yolk lipid peroxidation (ILP), and anti-AGEs activity, through the inhibition of the formationof fructosamine (IF), β-amyloid (Iβ), protein carbonylation (IC) and AGEs (IA). Polyphenolswere quantified by HPLC.Results: Maximum response ± SEM: For F 0.01 μg/mL: IF = 42 ± 4%, IC = 17 ± 2% and for10 μg/mL: IA = 38 ± 4%, Iβ = 67 ± 7%. For L 0.1 μg/mL: IF = 35 ± 2%, IC = 19 ± 2% andfor 100 μg/mL: IA = 26 ± 3%, Iβ = 63.04 ± 2%. The DPPH IC50 = 134.8 ± 14 μg/mL for Fand 34.67 ± 3 μg/mL for L. The ILP IC50 = 512.86 ± 50 μg/mL for F and 154.8 ± 15 μg/mL for L. By HPLC L presented the highest amounts of flavonoids and caffeoylquinic acids.F and L showed strong anti-AGEs activity, affecting the early stages of glycation at lowconcentrations and the late stages of glycation at high concentrations. The highest activityfor both F and L was seen in the IF and Iβ. F presented the highest anti-AGEs potency.L presented the highest antioxidant potency, which was related to the highest content ofpolyphenols.Conclusion: The fruits of I. paraguariensis could be a source of antioxidant and anti-AGEscompounds to be used with medicinal purposes or as functional food.