Metabolic Syndrome in female mouse: effect on fertility and ovarian angiogenesis

VELAZQUEZ, CANDELA; NICOLAS DALTON; DÉBORA COHEN; LEOPOLDINA SCOTTI; NATALIA PASCUALI; GONZALO OUBIÑA; LUCRECIA NEGRI MONTES; ADRIANA DE SIERVI; FERNANDA PARBORELL; ABRAMOVICH, DALHIA

Mar del Plata

Congreso; Reunión Anual de SOCIEDADES DE BIOCIENCIA; 2018

Sociedad Argentina de Investigaciones Clínicas (SAIC)

The MS is a set of disorders that include at least three of these pathologies: overweight, high blood pressure, high triglycerides, high glycemia and low HDL. Metformine (MET) is a drug taken mainly by patients that suffer diabetes type 2, and it has been observed that it is capable of improving fertility in sick women.As the age of getting pregnant has increased, the possibility of being affected by the effects of MS on women fertility is higher.Objective: To evaluate the ovarian quality, in vitro fertility, follicular development, and ovarian PDGFB levels in a MS model of female mouse. To study the effects of MET in the ovary.Methods: C57 females, four weeks old, were fed with a fat diet (FD) during 17 weeks. Control animals received a standard diet (SD). A group of FD also received MET. The estrous cycle was evaluated by vaginal cytology and the animals were sacrificed on week 17 after measuring glycemia levels. Serum was extracted to measure cholesterol and triglycerides and ovaries to obtain proteins. We finally analyzed the follicular dynamicAnother mouse group was administrated with 10 UI of eCG and 48 hs later 10 UI of hCG. After 16 hours, animals were sacrificed and the number of the ovulated oocytos was counted. We then made an in vitro fertilization and measured the percentage of fertilized oocytos. We also calculated how many fertilized oocytos could developed until blastocyst stage. Results: Animals fed with FD showed higher weight, cholesterol and triglycerides serum levels. We didn?t find any differences in the number of ovulated oocytes or fertilized oocytes between groups. The percentage of embryos that developed until blastocyst was lower in the FD group compared with the SD group and MET was able to reverse the effect. The FD group spent more time in diestro and less time in estro and the MET group spent more time in metaestro and less time in estro comparing with FD group. Also, MET raised ovarian PDGFB comparing it with the other groups. Finally, the follicular dynamic was altered in FD animals and MET improves it, raising the percentage of Luteal Bodies and reducing the percentage of atresic follicules. Conclusions: The MS alters the folicular dynamic and the ovarian quality. MET administration would have a beneficial effect in fertility in MS pathology.