POLYCYSTIC OVARY SYNDROME ALTERS THE ENDOCRINE PROFILE AND INCREASES HISTOMOR- PHOLOGICAL ALTERATIONS IN THE UTERUS OF AGED RATS IN THE LONG-TERM

BRACHO GS; IÑIGUEZ I; LOPEZ AF; ACOSTA MV; KASS LAURA; LUQUE ENRIQUE H; BOSQUIAZZO VERÓNICA L

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

This study aimed to investigate the long-term impacts of polycysticovary syndrome (PCOS) on rat uterine health. To achieve this, weevaluated: a) Levels of serum steroid hormones , b) determinationof uterine lesions incidence and multiplicity, c) evaluation of uterine collagen remodeling, and d) infiltration of eosinophils. PCOS wasinduced in female Wistar rats through sc injection of dehydroepi-androsterone (6mg/100g bw) from 21 to 40 days of age. The CON-TROL group received injections of sesame oil. Animals were eutha-nized at 18 months (CONTROL18 and PCOS18) and at 24 months(CONTROL24 and PCOS24). Blood samples were collected forthe quantification of sex steroids, and uterine were dissected andprocessed for histomorphological analysis. At 18 months, levels of17β-estradiol, progesterone and testosterone were comparable be-tween the groups, revealing distinct alterations in luminal epitheliumand glands. PCOS18 rats exhibited an increased in multiplicity ofglands with squamous metaplasia and gland conglomerates. Addi-tionally, elevated of collagen remodeling was observed in subepi-thelial stroma along with a reduction in the myometrium. Eosinophilinfiltration remained unchanged. At 24 months, PCOS24 rats dis-played lower serum progesterone compared to CONTROL24 rats(CONTROL24: 22.4 ± 6.2 vs. PCOS24: 5.9 ± 1.9 ng/mL, p