Regulation and role of Acyl-CoA Synthetase 4 in neurosteroidogenic cells

DATTILO, MELINA A.; HERRERA, LUCÍA M.; BENZO, YANINA; PRADA, JESICA G.; COHEN SABBAN, JUAN M.; GARCÍA, CORINA I.; PAZ, CRISTINA; MALOBERTI, PAULA M.

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

The brain is a neurosteroidogenic organ in which neurons, astrocytes and microglia express enzymes related to neurosteroidogenesis. Acyl-CoA synthetase4 (ACSL4) enzyme expression in the brain has been described. Although the role of ACSL4 in neurons and in the development of the nervous system has been studied, nothing had been described about its role in neurosteroidogenesis and its regulation in glia cells. Here we show that in primary astrocyte cultures incubatedwith1mM 8Br-cAMP for 12 h, ACSL4 mRNA levels are increased compared to untreated cells (p