REGULATION OF ACYL-COA SYNTHETASE 4 (ACSL4) EXPRESSION BY TRANSCRIPTIONAL AND POST TRANSCRIPTIONAL MECHANISMS IN BREAST CANCER CELLS

BENZO Y.; DATTILO M.; PRADA J.; HELFENBERGER K.; HERRERA L.; PODEROSO C.; MALOBERTI P.

Congreso; REUNION CONJUNTA SAIC SAI SAFIS 2018; 2018

Acyl-CoA synthetase 4 (ACSL4) is an enzyme that catalyzes acylCoA synthesis from long chain fatty acid, being arachidonic acid itspreferred substrate. ACSL4 levels correlate with aggressive phenotypein breast cancer. Its expression promotes tumor aggressivenessby increasing migration, proliferation and invasion. Theaim of this work is to describe transcriptional and post traductional mechanisms that could regulate ACSL4 expression in breast cancercell lines. We demonstrated by ChIP and by the use of a specific inhibitor that Estrogen-related receptor alpha is involved in ACSL4 transcriptional regulation. We studied the participation of proteosomal degradation. ACSL4 protein stability was tested on MCF-7 breast cancer cells with cycloheximide (CHX) treatment. Immunoblot showed a time-dependent decrease in ACSL4 levels after CHX treatment, significant at 2h of CHX (***p