Role of Acyl-CoA synthetase 4 in mitochondrial bioenergetics in breast cancer cells

BENZO Y.; DATTILO MA; HELFENBERGER K.; RINGELMANN SANCHEZ P.; MORI SEQUEIROS GARCIA MM; CASTILLO AF.; PODEROSO C.; MALOBERTI PM

Congreso; LXIV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

Acyl-CoA synthetase 4 (ACSL4) catalyzes acyl-CoA synthesis from long chain fatty acid, being arachidonic acid its preferred substrate. In breast cancer, ACSL4 promotes tumor aggressiveness by increasing migration, proliferation and invasion. In cancer cells, there is evidence of dysregulation of mitochondrial function, mitochondrial mass and subcellular spatial organization. The aim of this work is to study whether mitochondrial metabolism is regulated by ACSL4 in breast cancer cells. We evaluated levels of specific proteins in mitochondrial fraction of the MCF-7 stable breast cancer cell line overexpressing ACSL4 (MCF-7 tet-off/ACSL4) and control cells (MCF-7 tet-off/empty vector). We observed a significant increase in Complex III (p