Study and development of formulations based on microemulsion for the delivery of ampicillin

MICAELA PONCE PONTE; CAROLINA ALOISIO; MARCELA LONGHI

Cordoba

Congreso; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); 2021

Ampicillin (AMP) is one of the first-line semisynthetic penicillin, due to its broad spectrum and efficacy against gram-positive and gram-negative bacteria. In Biopharmaceutical Classification System (BCS), AMP is categorized as Class III, based on the high solubility and low permeability of the highest dose. The purpose of this study was to improve the permeability of AMP by developing an oil-in-water microemulsion. Pseudoternary phase diagrams were constructed to identify the proper compositions to form microemulsions (ME). A Polysorbate 80:Polyethylene glycol 400 and Polysorbate 80:Triton X-100 mixtures were selected as Surfactant, ginger and eucalyptus oils constituted the oil phase and water was used as the aqueous phase. The droplet sizes of the internal phase of the ME were measured using the Dynamic Light Scattering. Besides, the ME were also characterized by conductivity determination. Later, the amount of AMP incorporated into the systems was measured using HPLC. The formulation that presented the highest amount of AMP incorporated (22.21 mg/ml) consist of 7% polysorbate 80, 7% TritonX-100, 6% eucalyptus oil, and 80% water. These results represent a system with the potential to improve the efficacy of AMP administrated orally.