EFFECTS OF OXIDIZED LOW-DENSITY LIPOPROTEIN (OXLDL) ON PROSTATIC STROMAL CELLS DERIVED FROM PATIENTS WITH BENIGN PROSTATIC HYPERPLASIA

ROLDÁN GALLARDO F.F.; CUELLO RUBIO M.; LOPEZ SEOANE M; MALDONADO CA; QUINTAR AA

Buenos Aires

Congreso; REUNION ANUAL DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

(154) EFFECTS OF OXIDIZED LOW-DENSITY LIPOPROTEIN (OXLDL) ON PROSTATIC STROMAL CELLS DERIVED FROM PATIENTS WITH BENIGN PROSTATIC HYPERPLASIARoldán Gallardo FF1,2, Cuello Rubio MM1,2, López SeoaneMR3, Maldonado CA1,2, Quintar AA1,21. Universidad Nacional de Córdoba, Facultad de CienciasMédicas, Centro de Microscopía Electrónica. Córdoba, Argentina.2. Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET), Instituto de Investigaciones en Ciencias de laSalud (INICSA). Córdoba, Argentina. 3. Sanatorio Allende, Sede Nueva Córdoba. Córdoba, Argentina.Benign Prostatic Hyperplasia (BPH) affects elderly men, being theresult of an excessive and uncontrolled cellular proliferative process of both epithelial and stromal prostatic compartments. Novelevidence suggests that dyslipidemias and other factors of the Metabolic Syndrome are associated to BPH progression and aggressiveness. However, little information is available about the pathogenicmechanisms promoting prostatic growth in atherogenic contexts.We therefore aimed to analyze in vitro the effects of OxLDL on primary cell cultures. Prostatic stromal cells, surgically harvested frompatients with BPH (n=3), were isolated, cultured, and stimulated withOxLDL (20 µM or 100 µM, simulating an atherogenic state) or vehicle for 24h and 48h.OxLDL induced cell proliferation, as assessed by BrdU incorporationand Ki67 immunocytochemistry, mainly at lower concentrations andin a time-dependent manner (p