GIMÉNEZ MARTÍNEZ Pablo Darian
congresos y reuniones científicas
Antimicrobial activity of phenolic extract of apple pomace against Paenibacillus larvae and its toxicity on Apis mellifera
GIMÉNEZ MARTÍNEZ PABLO DARIAN; RAMIREZ AMBROSI, M; ARREDONDO, DANIELA; GALLO B,; ALONSO-SALCES ROSA MARIA; ANTUNEZ, KARINA; MAGGI MATIAS; FUSELLI SANDRA
Congreso; Eurbee 8; 2018
Apis mellifera populations are threatened by different biotic and abiotic stressors that affect the bee survival. Pathogensand parasites are among the most important factors that cause mortality of bees. Paenibacillus larvae, the causativeagent of American foulbrood, is one of the most important bacterial pathogens that affect bee health. The use of antibiotics,particularly tetracycline hydrochloride (OTC), is the most common method for prevention and treatment ofinfected colonies. The extended use of synthetic drugs is known to generate several problems, including the presence ofchemical residues in the beehive products (honey, pollen and wax), which eventually may even affect consumer health.However, this application can increase the risk of occurrence of resistant strains. An ecological alternative is the use ofvegetal extracts containing bioactive compounds, such as polyphenols. The aim of the present study was to evaluate theantimicrobial activity of phenolic extracts of apple pomace, coming from the cider industry, against P. larvae by the brothmicrodilution method. Besides, the toxicity of the extracts on A. mellifera was evaluated using the complete exposuremethod. All extracts contained from 1022 to 9364 mg/g of total phenolic contents, determined by HPLC-DAD, showedantimicrobial activity against P. larvae genotype ERIC I, ranging from 20 μgmL-1 to 150 μgmL-1.Toxicity assays of applepomace extracts on adult bees exhibited a maximum mortality of 18% after 48h. Although some extracts analyzed inthe present study showed higher MIC values than OTC, apple pomace extracts are still promising, since being naturalproducts they would lack the problems that synthetic antibiotics have, including the presence of residues in the beehiveproducts and the occurrence of resistant strains. Furthermore the reuse of agro-industrial residues such as apple pomacethat are generated in great quantity causing environmental problems, is being paid attention by governments andenvironmental protection institutions.