Short communication: A potential role of tannins in the control of American Foulbrood

GIMÉNEZ MARTÍNEZ, PABLO DARIAN; MAGGI, MATÍAS; FUSELLI, SANDRA R.

SPANISH JOURNAL OF AGRICULTURAL RESEARCH

SPANISH NATL INST AGRICULTURAL & FOOD RESEARCH & TECHNOLO

Lugar: Madrid; Año: 2021 vol. 19

1695-971X

Aim of study: to evaluate the use of tannins extracts in the control of the American Foulbrood pathology; and to investigated if these extracts present levels of toxicity on Apis mellifera.Area of study: Paenibacillus larvae Strains C1 and C2 were from Balcarce, province of Buenos Aires (37°52?S-58°15?W), strain C6 from Rio Cuarto, in Cordoba province (33°08′00″S 64°21′00″O) and strain C9 from Concordia in Entre Rios province (31°23′32″S 58°01′01″O). Bees larvae used for toxicological assays were collected in Santa Paula experimental apiary, Mar del Plata (National Route 226, Km 10, Argentina, (37°55′48″S 57°40′59″O)), belonging to the Centro de Investigación en Abejas Sociales (CIAS - IIPROSAM) from UNMdP.Material and methods: The Minimal Inhibitory Concentration (MIC) of five different tannin extracts were obtained by agar diffusion method on four P. larvae strains; using the MIC value, the toxicity test on Apis mellifera larvae was performed afterwards.Main results: The MIC value is in the range of 6.9 to 898.6 µg/mL. Three tannin extracts did not show toxicity against bee larvae; however those that were fed with the latter showed a significant increase in weight.