BECAS
GIMÉNEZ MARTÍNEZ Pablo Darian
artículos
Título:
Short communication: Antimicrobial activity of indoleacetic, gibberellic and coumaric acids against Paenibacillus larvae and its toxicity against Apis mellifera
Autor/es:
SZAWARSKI, NICOLÁS; GIMÉNEZ MARTÍNEZ PABLO DARIAN; MITTON, GIULIA; NEGRI, PEDRO; MEROI ARCERITO, FACUNDO; MOLINÉ, MARIA P.; FUSELLI, SANDRA; EGUARAS, MARTÍN; LAMATTINA, LORENZO; MAGGI, MATÍAS
Revista:
Spanish Journal of Agricultural Research
Editorial:
Spanish Journal of Agricultural Research
Referencias:
Año: 2020 vol. 18
Resumen:
Aim of study: To explore three isolated phytomolecules: indoleacetic acid (IAA), gibberellic acid (GA), and the secondary me- tabolite p-coumaric acid (CUM): (1) evaluating their toxicity against Apis mellifera larvae and adults under controlled conditions in the laboratory; (2) searching for antimicrobial activity against Paenibacillus larvae.Area of study: Honey bee larvae and adults were collected from the experimental apiary of the ?Centro de Investigación en Abejas Sociales (CIAS)? (-37.9348798, -57.682817), Institute of the National University of Mar del Plata (UNMdP), Argentina.Material and methods: Paenibacillus larvae strains were isolated from beehives from different provinces of Argentina (Buenos Aires, Córdoba and Entre Ríos) showing clinical symptoms of the American foulbrood. All strains (S1, S2, S3, S4) were genotypi- cally identified using PL5 and PL4 primers and characterized as genotype ERIC1. Then standard essays were performed to determined toxicity of phytomolecules in honey bees and antimicrobial activity through the broth microdilution method.Main results: The diet with GA, IAA and CUM did not present toxic effects in larvae or adult bees, and only CUM showed an- timicrobial activity against P. larvae. In this study, we obtained in vitro values of MNIC (minimum non-inhibitory concentration) of 500 μg mL-1 and a MIC (minimum inhibitory concentration) of 650 μg mL-1 for CUM.