SELECTION OF PARENTS AND ESTIMATION OF GENETIC PARAMETERS USING PHENOTYPIC, BLUP AND MOLECULAR METHODS FOR LENTIL (LENS CULINARIS MEDIK) BREEDING PROGRAM IN ARGENTINA

BERMEJO, C; CAZZOLA, F; MAGLIA, F; COINTRY, E

EXPERIMENTAL AGRICULTURE

CAMBRIDGE UNIV PRESS

Lugar: Cambridge; Año: 2018

0014-4797

The most important objective of lentil breeding programs is to develop new genotypes that are genetically more productive. Besides, it is necessary that the varieties obtained have short flowering cycles to allow the later sowing of summer crops. Selection is based through phenotypic means but should be based on the genetic or breeding values because quantitative traits are often influenced by environments and genotype?environment interaction. The objectives of this study were to compare the efficiency of the methods based in phenotypic mean values, breeding values and molecular markers, identify the plants with the highest merit and estimate genetic parameters to know the genetic control of morphological traits in lentil using Best Linear Unbiased Prediction (BLUP). Twenty five Recombinant Inbred Lines (RILs) from six F4 families were selected based on precocity and high yields and were tested in four environments for important quantitative traits. The analysis of variance showed significant differences between genotypes, environments and genotype-environment interaction for all the traits. The genotype main effect plus genotype by environment interaction (GGE) biplot analysis established two mega-environment, the third showed adverse climatic conditions. Seven macrosperma and two microsperma type RILs were selected. Based on average ranking from breeding values and the molecular data using SRAP (Sequence-Related Amplified Polymorphism) the same genotypes were selected. Genotypic coefficients of variation, heritabilities across and by environment and genetic correlation coefficients using BLUP were obtained. BLUP could replace the phenotypic and molecular analysis methods because the selection process was simpler, with lower cost and more accurate. The breeding values would give a better ranking of the genetic value of the parents than would their phenotypic value and, therefore, the selection efficiency would be enhanced and the genetic gain more predictable. The selected genotypes could become in potential commercial varieties or used as parental in future hybridization programs.