CONICET | Buscador de Institutos y Recursos Humanos

Chickpea blight, a fungal disease caused by Ascochyta rabiei, is the most damaging disease in chickpea fields worldwide. Since its first report in Argentina in 2012, has caused yield losses up to 100%. The exploratory study of the proteomics in the plant-pathogen interaction could be a potential tool to identify candidate proteins to improve chickpea defense responses against Ascochyta rabiei. Thus, they could be helpful to identify candidate genes and their further selection in chickpea genetic improvement programs. This work was aimed to find some differentially regulated proteins related with chickpea defenses in the plant-pathogen interaction. A susceptible chickpea genotype, Chañaritos S-156, and virulent isolated of Ascochyta rabiei RCB were used for this assay. A sample of three independent total proteins extractions were obtained of each treatment: inoculated vs non inoculated plants. The extracts were analyzed by Free label Quantification. More than 900 proteins were identified in each sample. Differentially expressed proteins were determined through with a student test (T-test), these results are represented in a volcano plot made in the Perseus software using p=0.05 as a cut-off value. Volcano plot shows 33 proteins significantly upregulated, and 80 proteins downregulated in the inoculated condition vs. non inoculated. Of those up-regulated, 24 have been cited before as related to plant defenses to biotic stresses and 15 of them belong to PR proteins. This is the first attempt to the understanding at proteomic level on the chickpea blight pathosystem in Argentina.It contributes to the understanding of Ascochyta rabiei-Cicer arietinum interaction and can contribute to identifying key factors for chickpea genetic improvement.

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