Homeostasis loss in VIP deficient mice is associated with altered regulatory T cells recruitment

GALLINO LUCILA; HAUK VANESA; GRASSO ESTEBAN; PALLIGAS MARCOS; SARACO NORA; BERENSZTEIN ESPERANZA; PASCUALI NATALIA; PARBOREL FERNANDA; WASCHEK JAMES; PEREZ LEIROS CLAUDIA; RAMHORST ROSANNA

Simposio; 8th Latin American Symposium on Maternal-Fetal Interaction and Placenta (VIII SLIMP); 2019

Objetive: In order to have a successful pregnancy, it is required a balance between proinflammatory and tolerogenic mediators. Since VIP (vasoactive intestinal peptide) is a master regulator associated with Tregs induction and recruitment, we aim to understand the relevance of VIP in normal pregnancy with focus on the Tregs. Methods: We used two mice models: VIP Knockout and Foxp3-IRES-GFP, both in a C57 background. The serum and tissues obtained were analyzed by flow cytometry, RT-PCR and histological staining. We also performed Foxp3-adoptive cell transfer and migration assays.Results: On the day of estrus we found histological differences between the ovaries and uterus of a WT mice vs VIP knockout mice (-/-). In uterus, these changes were accompanied with undetectable levels of expression Foxp3 in the (-/-) group, higher expression of RORγt and a decrease in IL-10 (p