VIP induces an immunosuppressant microenvironment in the maternal-fetal interface of non obese diabetic (NOD) mice and improves pregnancy outcome

CALO GUILLERMINA; HAUK VANESA; AZZAM SOFIA; GALLINO LUCILA; FRANCHI ANA; RAMHORST ROSANNA; PEREZ LEIROS CLAUDIA

Simposio; V SLIMP - IV LASRI Meeting 2013; 2013

Background: Immune homeostasis loss in maternal interface of non obese diabetic (NOD) mice was related to autoimmune prone background. VIP has anti-inflammatory, tolerogenic and relaxing effects so its ability to promote an immunosuppressant microenvironment and improve pregnancy outcome was explored. MethodsPrediabetic NOD mice were used and RTPCR and immunohistochemistry of d9 explants of implantation sites performed. (Protocol aproved School of Sciences Ethics Committee).Results:VIP and VPAC2 receptor expression was detected in pregnant uterus and deciduas. VIP treatment of implantation site explants inhibited prostaglandin synthesis and increased VPAC2, TGF-β, Foxp3 and IL-10 expression. Sites with resorption processes presented lower VIP and suppressant mediator expression than viable sites, with increased COX-2 activity, IL-17 and RORT expression. Pregnant NOD mice treated with VIP at d6 presented higher number of viable embryos, an even distribution along the horns, and higher IL-10, TGF- β and Foxp3 expression. Conclusion: VIP modulates inflammatory signals at the early maternal-fetal interface of NOD mice improving pregnancy outcome.