INVESTIGADORES
SESMA Juliana Ines
artículos
Título:
A simple method for Isolating DNA of High yield and quality from cotton (gossypium hirsutum L.) leaves
Autor/es:
HUGO PERMIGEAT; VALERIA ROMAGNOLI; JULIANA SESMA; RUBEN VALLEJOS
Revista:
PLANT MOLECULAR BIOLOGY REPORTER
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 1998 vol. 16 p. 89 - 93
ISSN:
0735-9640
Resumen:
An easy, reproducible and fast procedure to isolate DNA from cotton leaves is described. The addition of 0.5 M glucose in the extraction buffer avoids browning by polyphenolic compounds and improves the quality of DNA for molecular analysis. The DNA yield ranged between 150?400 g per gram of fresh tissue. DNA was suitable for digestion by restriction enzymes and amplification by Taq DNA polymerase.
