STUDY AND COMPARISON OF BIOFILM FORMATION BY REGIONAL Clostridium spp STRAINS

CELIA NOEMÍ PEREZ; RODRIGO GUERRA; VILLA MARIA CECILIA; PATRICIA STAGNITTA

Mendoza

Otro; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016

Clostridium perfringens is an anaerobic gram-positive pathogen, causative agent of numerous diseases, biofilm forming recently informed. C. septicum, like C. perfringens is an opportunistic pathogen causative agent of myonecrosis. C. chauvoei is the causative agent of the stain, an infectious disease considered endemic in San Luis province. Bacterial biofilms are communities of cells adhered to surfaces, immersed in a matrix of extracellular that protects the antibiotic attack and increases its resistance. The aim of this work was characterize phenotypically the biofilm formation of C. perfringens, C. septicum and C. chauvoei and compare with our preliminary studies on different surface. Strains used: C. perfringens ATCC 3624, C. septicum ATCC 12464 and C. chauvoei ATCC 10092. For the biofilm formation, Petri dishes with OMA (optimized medium for anaerobes) containing coverslips were cultured at 37°C in anaerobic conditions for 24, 48 and 72 h. For the planktonic and sessile cells analysis, Gram, 0,1% crystal violet colorations and scanning electron microscopy (SEM) were used. Quantification of biofilm and planktonic cells was performed by Abs 570/DO580 relationship. For statistical analysis Student's test was used. At 24 h a good production of biofilm was obtained, their proportion increase to 48 and 72 h for 3 spices. The Abs570/DO580 at 48 h of culture showed that the biofilm formation efficiency in glass surface was C. perfringens> C. septicum> C. chauvoei (p≤0,05). The same was observed with optical microscopy and SEM. It was different on polystyrene plastic (C. septicum> C. perfringens> C. chauvoei). Influence of different surfaces on biofilm formation and affinity for bacterial adhesion to various materials was evidenced. C. septicum and C. chauvoei have a good ability to form biofilms, data not shown before. This is very important in the pathogenesis and resistance provided by the biofilm and difficult the diseases treatment caused by these microorganisms.