Novel sequencing type of an Enterobacter cloacae complex strain with the potential to become a high-risk clone st 298

C. A. KNECHT; A. S. GAMBINO; A. C. LEGUINA; ÁLVAREZ VE; PRACK MC CORMICK B; MASSÓ, M; GONZALES MACHUCA A; CARRERA PÁEZ LC; PIEKAR M; CAMPOS, J; FOX, B; CAMICIA G; AYALA NUÑEZ, T; VARGAS CV; ALONSO, FERNANDO M.; DONIS, N; FERNANDEZ CANIGIA L; CENTRÓN D; QUIROGA MP

Congreso; International Union of Microbiological Societies (IUMS) Congress; 2022

Enterobacter cloacae complex (ECC) has awakened interest recently because of its increasing resistance to carbapenems codified by several genes all over the globe. Even though there are some sequence types (STs) which represent high-risk clones, there is substantial clonal diversity in the ECC. This work aimed to perform whole-genome sequencing (WGS), genomic analysis, and phylogenetic studies of a Klebsiella pneumoniae carbapenemase (KPC) -producing multidrug-resistant (MDR) ECC isolate from Argentina.We analysed the genome of an MDR KPC-producing ECC strain isolated from a urine sample from a patient in a hospital in Argentina. The WGS was done by Illumina MiSeq-I (Illumina, San Diego, CA). The genome was assembled with SPAdes 3.9.0, and annotated with PROKKA, RAST, and Blast. Plasmids were identified with PlasmidFinder. Antibiotic resistance genes were detected using RESfinder, CARD, and Blastn. STs were identified with pubMLST.