PTHrP PROMOTES AN AGGRESSIVE BEHAVIOR OF CRC CELLS THROUGH ITS ACTION ON TUMOR AND STROMAL CELLS

NOVOA DIAZ MARÍA BELEN; CARRIERE PEDRO; BIRKENSTOK CINTIA; PICARDI GONZALO; GÓMEZ LUIS; GIGOLA GRACIELA; ZWENGER ARIEL; CALVO NATALIA; GENTILI CLAUDIA

Mar del Plata

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2023

.The interaction of several cells and molecules in the tumor microenvironment (TME) promotes tumor-aggressive features. Previously we found that parathyroid hormone-related peptide (PTHrP), acytokine from TME, promotes an aggressive phenotype in HCT116colorectal cancer (CRC) cells and that conditioned medium fromPTHrP-treated HMEC-1 endothelial stromal cells (CM) induces epithelial to mesenchymal transition (EMT) in CRC cells. The aim ofthis work is to further investigate the PTHrP effects on CRC and itsTME. HCT116 cells were treated with the CM. Western blot (WB)analysis showed that CM modulates the expression and activationof Met, a receptor related to CRC (p