OPTIMIZATION OF THE SIMULTANEOUS SACCHARIFICATION AND FERMENTATION STRATEGY FOR 2G ETHANOL PRODUCTION IN PINE SAWDUST BIOREFINERY

MENDIETA, CAROLINA M.; KRUYENISKI, J.; FELISSIA, FERNANDO E.; AREA, MARÍA. C.

Girona

Congreso; CIADICYP 2022 Iberoamerican Congress on Pulp and Paper Research; 2022

Universidad de Girona

The objective of this work is evaluate the second-generation bioethanol (2G) production from a pine sawdust soda-ethanol pulp by simultaneous hydrolysis and fermentation (SSF) to obtain the best conditions for scaling up the process [1]. Factorial experimental designs with central points were employed to evaluate the combined influence of enzymatic load, temperature, and time on bioethanol yields. The Statgraphics Centurion software was used to assess the experimental design results at a 95% confidence level. The pulp used has the following chemical composition: 80.18% Glucans, 7.20% Xylans, 0.29% Galactans, 8.40% Mannans, and 3.67% Lignin. Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. In addition, contains a strain recovery technique to reactivate yeast colonies used in previous works to preserve them and be used in this work as well as in future works. This study allowed defining adequate conditions for each processing stage, which can be adjusted to achieve maximum bioethanol production. The calculated fermentation yield values fit the experimental values, establishing the validity of the obtained models. Fermentation yields optimal values were 63.2% and 81.9% for Models I and II, respectively. The best conditions found by the experimental designs were 30 FPU g-1 glucans, 39°C and 24 h for Model I, and 30 FPU g-1 glucans, 35°C and 72 h for Model II.