BECAS
PACINI MarÍa Florencia
congresos y reuniones científicas
Título:
EARLY TARGETING OF MYELOID-DERIVED SUPPRESSOR CELLS IMPROVES THE PROTECTIVE CAPACITY OF TSf-ISPA VACCINE AGAINST TRYPANOSOMA CRUZI
Autor/es:
JUAN CRUZ GAMBA; ESTEFANÍA PROCHETTO; GIULIANA LUPI; IVÁN BONTEMPI; FLORENCIA GONZÁLEZ; FLORENCIA PACINI; CAROLINA PONCINI; MÓNICA VERMEULEN; ANA ROSA PÉREZ; IVÁN MARCIPAR; GABRIEL CABRERA
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión de Sociedades de Biociencias 2020; 2020
Institución organizadora:
SAI-SAIC-SAFIS
Resumen:
Introduction: We have previously reported that immunization witha trans-sialidase-based antigen (TSf) formulated with a cage-likeparticle adjuvant (ISPA) confers protection against Trypanosomacruzi (T.cruzi). It has been shown that myeloid-derived suppressorcells (MDSCs) notably increase in several organs during mice acuteinfection.Aim: to study whether MDSC depletion could further improve theefficacy of the TSf-ISPA vaccine candidate against T.cruzi.Methods: 3 subcutaneous doses of TSf-ISPA were given to BALB/cmice before intraperitoneal infection with 1000 Tulahuen trypomastigotes.5-fluorouracil (5FU) (50mg/kg) was given at different times(prior or during infection) to deplete MDSCs. Parasitemia was monitoredat days 14 and 21 postinfection (p.i.). Survival was assesseduntil day 40 p.i. Flow cytometry was used to measure the % andnumber of MDSCs (CD11b+ GR-1+), CD4 and CD8 T cells, B cellsand CD11chigh dendritic cells in the spleen after 5FU treatment.Results: Survival after infection was always higher in TSf-ISPA-immunizedand infected mice (TSf-ISPA Tc+ group) than control infectedPBS-treated mice (PBS Tc+ group) (p
