INTEGRATED PHARMACOLOGICAL EVALUATION OF THE COMBINATION OF SYNTHETIC ANTHELMINTICS AND BIOACTIVE PHYTOCHEMICALS: IN-VITRO AND IN VIVO STUDIES

MIRÓ, M.V.; LUQUE, S.; LLOBERAS, M.; COSTA-JUNIOR, L.M.; LANUSSE, C.; VIRKEL, G.L.; LIFSCHITZ, A.L.

Mar del Plata

Congreso; 51° REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); 2019

In a context of increasing anthelmintic resistance, combination of synthetic anthelmintics with bioactive phytochemicals may be a pharmacological tool for improving the nematode control in livestock. The coadministration of natural compounds and anthelmintics may lead to kinetic/dynamic interactions. This work evaluated the drug-drug interactions between synthetic anthelmintics and bioactive phytochemicals at the metabolism and drug transport level. The impact of these interactions on drug efficacy was also studied. Trial 1 include in vitro and in vivo the combination of thymol (TML) and albendazole (ABZ). The stability of TML (800 µg/ml) in sheep ruminal content and its effects (5 mM) on the metabolism of ABZ in sheep liver microsomes were evaluated. The in vivo plasma concentrations and efficacy of the combination were studied in lambs naturally infected with resistant nematodes. TML was stable in sheep ruminal content and inhibited (P