CARVONE MODULATES IN VITRO AND IN VIVO THE KINETIC BEHAVIOUR AND EFFICACY OF ABAMECTIN

MIRÓ, M.V.; LUQUE, S.; CARDOZO, P.; LLOBERAS, M.; CANTÓN, C.; COSTA-JUNIOR, L.M.; LANUSSE, C.; VIRKEL, G.L.; LIFSCHITZ, A.L.

Utah

Congreso; 65th Annual Meeting of The American Association of Veterinary Parasitologists; 2020

The search of novel strategies to control gastrointestinal nematodes in ruminants is a concern considering the increasing of anthelmintic resistance. Bioactive phytochemicals may contribute to improve parasite control by enhancing the effect of existing anthelmintic drugs. This work assessed the in-vitro and in-vivo pharmacological interaction and the in-vivo efficacy of abamectin (ABM) combined with the plant-derived compounds carvone (CNE), in lambs naturally infected with resistant gastrointestinal nematodes. At first, the modulation of P-glycoprotein (P-gp) by CNE was assessed using the intestinal explant model. Rhodamine-123 (Rho123) and ABM were used as substrates to measure their accumulation in cattle ileum in presence or absence of CNE. For the in vivo assay, twenty-eight (28) lambs were allocated into three (3) experimental groups. Each group was treated orally with either ABM (0.2 mg/kg), ABM in combination with CNE (100 mg/kg, four doses every 24 h) or remained as untreated control. Blood samples were collected between 0 and 168 h post-treatment and plasma levels of both compounds were determined by HPLC. Individual fecal samples were collected on days -1 and 14 post-treatment to perform the fecal eggs count reduction test. The presence of CNE increased significantly (P