PHARMACOLOGICAL CHARACTERIZATION OF MONOTERPENES WITH POTENTIAL NEMATODICIDAL ACTIVITY IN RUMINANTS

MIRÓ, VICTORIA; COSTA JUNIOR, LIVIO; LLOBERAS, MERCEDES; CARDOZO, PATRICIA; LANUSSE, CARLOS; VIRKEL, GUILLERMO; LIFSCHITZ, ADRIAN

Ilheus, Bahia

Congreso; XXI Congresso Brasileiro de Parasitologia Veterinária; 2022

Sociedade Brasileira de Parasitologia Veterinária,

The search for novel alternatives to control gastrointestinal nematodes (GI) in ruminants is relevant in a scenario of increased anthelmintic resistance. The use of monoterpenes may enhance the effectiveness of existing synthetic anthelmintic drugs. However, it is necessary to study the potential pharmacological interactions and the fate of these compounds after their administration to ruminants. This work evaluated in vitro the effects of monoterpenes on the processes of drug metabolism and intestinal accumulation and the in vivo pharmacokinetics interactions between the best "candidate” and synthetic anthelmintics. In Phase 1, the intestinal accumulation of Rhodamine 123 (Rho123), a P-glycoprotein substrate, was studied in cattle ileum explants in the presence or absence of carvone (CNE), geraniol (GNL) and citral (CTL). The effect of CNE, GNL and CTL on flavin-containing monooxygenase (FMO) and cytochrome P450 (CYP) dependent metabolism was assessed in sheep liver microsomes measuring specific enzyme activities. The presence of CNE and GNL increased the accumulation of Rho123 in the ileum explants by 66 % and 46 % respectively (P