BACTERIAL INACTIVATION BY THE EFFECT OF NANOCOMPOSITE BASED ON ZINC OXIDE NANOPARTICLES

PEREYRA, JESICA YANINA; ACEVEDO, DIEGO FERNANDO; YSLAS, EDITH INES; BARBERO, CESAR ALFREDO

Cancum

Congreso; XXVII INTERNATIONAL MATERIALS RESEARCH CONGRESS; 2018

The Sociedad Mexicana de Materiales

In this work, the synthesis, characterization and application as bactericidal material of the nanocomposite is shown. The hydrogel was synthesized by copolymerization between N-isopropylacrylamide (NIPAM) and hydroxiethylmetha-acrylate (HEMA) (PNIPAM-co-10%HEMA). Zinc oxide nanoparticles (NPsZnO) were synthesized insitu inside of the biocompatible hydrogel the precursor salt zinc nitrate. The zinc nitrate was allowed to diffuse inside the hydrogel for 24 hours. Then the swelling hydrogel was immersed in KOH solution and then the nanoparticles were obtained by using microwave. The antimicrobial activity of the nanocomposite was evaluated using a Gram negative (-)bacterium as the Pseudomonas aeruginosa model. This action was studied by means of the diffusionmethod in agar, which was evidenced by the formation of inhibition halos. The size of the inhibition halos increases whit the incubation time (24-48 h) suggesting an important antibacterial effect of the nanocomposite. From the results of viability and inhibition halos using as a control group the hydrogeland as a group treated the nanocomposite at different incubation times a marked antibacterial action of the Zinc oxide nanocomposite (PNIPAM-co-10% HEMA) dependent on time was observed. In conclusion, these results reveal that zinc oxide generated inside of PNIPAM-co-10%HEMA can be used as intelligent hydrogel to kill bacteria.