Luminal immunoglobulin G recognizes specific microbiota from patients with Inflammatory Bowel Disease (IBD)

AME RM; REVIGLIONO JI; BALDERRAMO DC; ROMAGNOLI, PA

Santiago

Taller; Inflammation, Cancer and Intestinal Mucosal Immunology (ICIMI); 2023

IUIS-SMI-ALAI-LAMIG-ASOCHIN

The intestinal mucosa is continuously exposed to microorganisms from microbiota that influence the development of its immune system (IS) with its cellular and humoral responses. Inflammatory Bowel Disease (IID), which mainly includes Crohn's Disease (CD) and Ulcerative Colitis (UC), is characterized by an excessive response from the IS of the intestinal mucosa towards the microbiota. One of the components of the IS is the immunoglobulin G, whose role in the lumen is to protect the intestinal mucosa from possible infections with pathogenic microorganisms. It is unknown how the composition of the microbiota is altered in patients with IBD in our community compared to healthy individuals and if these possible changes promote intestinal inflammation. To answer this question, we designed a study to investigate the hypothesis that luminal IgG identifies bacteria involved in the pathogenesis of IBD. So far, 28 patients with IBD and 15 healthy controls were recruited, of which the epidemiological characteristics found are described here and the separation with magnetic beads is presented as a preliminary approach to enrich bacterial species of the microbiota as a step prior to its sequencing. Data obtained from a patient with UC show results comparable to those reported in other regions of the world. We believe that the recruited population and the microbiota separation technique utilized in our approach will allow us to find new tools for the diagnosis and treatment of patients with IBD.