MOLECULAR AND PROTEOMIC ANALYSIS OF PSYCHROTOLERANT Brevundimonas sp. CI19

ALVARENGA, ADRIANA; SADAÑOSKI, MARCELA ALEJANDRA; ZAPATA, PEDRO DARÍO; VILLALBA, LAURA; AMOROSO, MJ

Rosario

Congreso; L Congreso SAIB 2014; 2014

SAIB

Brevundimonas sp. Ci19 was isolated from seawater samples from sub-Antarctic marine ecosystems. In previewstudies showed production of a-L-rhamnosidase with a broad range of properties that allows its application in severalbiotechnological processes. The aim of this work was to analyze the differential protein expression byBrevundimonas sp. Ci19 in the presence of rhamnose or glucose as only carbon source under cold-adapted conditionsby two-dimensional polyacrylamide gel electrophoresis. Simultaneously, primers were designed for amplification ofa-L-rhamnosidase sequence. For proteomic analysis, the bacteria were incubated in LBM medium containing glucoseor rhamnose, during 48 h at 15°C and 200 rpm. The cells were recollected and broken using press French. Thesupernatants of the cell lysates were used as protein samples. Two-dimensional gel electrophoresis of the wholeproteins pattern of Brevundimonas sp. Ci19 revealed protein differential expression by rhamnose and glucosepresence. Sequencing by MALDI-TOF of the more relevant proteins suggested that different regulation schemes areinvolved in response to carbon source. In relation of molecular study, 542 bp were amplified with designed primersand the sequence presented 57% identity to the conserved domain pfam05592, belonging to the superfamily cl18469of rhamnosidases.