Isolation and selection of endophytic spore-forming bacteria with plant growth promoting properties isolated from Ilex paraguariensis St. Hil. (yerba mate)

LACZESKI, MARGARITA; ANDREA ONETTO; ILIANA JULIETA CORTESSE; GISELA MALLOZZI; CASTRILLO, MARÍA LORENA; BICH, GUSTAVO; FERMIN GORTARI; ZAPATA, PEDRO DARÍO; OTEGUI, MÓNICA BEATRIZ

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2020 p. 1 - 20

0001-3765

Yerba mate (Ilex paraguariensis St. Hil.) is a species native to the subtropicalregions of South America. Despite being an important crop for the region, there are fewstudies on the use of microorganisms to improve the growth of seedlings in the nurserystage. The objective of this study was to isolate spore-forming endophytic bacteria withplant growth promoting properties associated with yerba mate seedlings and determinetheir phytobeneficial effect under controlled laboratory conditions. Isolates were selectedbased on their sporulation capacity and evaluated for in vitro plant growth promotingproperties (nitrogen fixation, phosphate solubilization, production of siderophores andsynthesis of indolic compounds). Yerba mate seedlings were inoculated with the mostpromising isolates, which were identified via analyses of the sequence of their 16S rDNAgene as Bacillus circulans (12RS3) and Bacillus altitudinis (19RS3, T5S-T4). After 120 daysplants showed higher root dry weight when inoculated with isolate 19RS3 and highershoot dry weight with 19RS3 and T5S-T4. In conclusion, further studies to determine theability of these isolates to adapt to the climatic conditions and to survive amidst thenative soil microflora in yerba mate cultivated native soils, will be crucial for developingsuch strains as biofertilizer.