INVESTIGADORES
ZAPATA Pedro Dario
artículos
Título:
KH 2 PO 4 improves cellulase production of Irpex lacteus and Pycnoporus sanguineus
Autor/es:
RODRÍGUEZ, MARÍA DANIELA; ALONSO PAIVA, IVÁN MATHÍAS; CASTRILLO, MARÍA LORENA; ZAPATA, PEDRO DARÍO; VILLALBA, LAURA LIDIA
Revista:
Journal of King Saud University - Science
Editorial:
Elsevier
Referencias:
Año: 2018 vol. 31 p. 434 - 444
ISSN:
1018-3647
Resumen:
The optimization of cellulase production by Irpex lacteus and Pycnoporus sanguineus was investigated. Fractional factorial design was conducted to determine significant variables and interactions. Response surface methodology was applied through Box-Behnken design to determine the optimum level of eachfactor on cellulase production. The optimal conditions of culture media were (g/L): for I. lacteus CaCl2.2(H2O) 0.3, MgSO4.7(H2O) 0.3 and KH2PO4 3, while for P. sanguineus was CaCl2.2(H2O) 0.1, MgSO4.7(H2O) 0.2 and KH2PO4 9. This optimized medium improved cellulase production, especially β-glucosidase activity values for I. lacteus. KH2PO4 was found in this work to be a useful mineral compound for cellulolytic production.
