Exploring Agaricomycetes from the Paranaense rainforest (Misiones, Argentina) as an unconventional source of fibrinolytic enzymes

ACOSTA, GABRIELA ALEJANDRA; FONSECA, MARÍA ISABEL; FARIÑA, JULIA INÉS; ZAPATA, PEDRO DARÍO

MYCOLOGIA.

ALLEN PRESS INC

Año: 2022 p. 1 - 12

0027-5514

Fungal fibrinolytic enzymes, secreted by some Agaricomycetes, are recognized as importantthrombolytic agents due to their ability to rapidly dissolve thromboembolic clots. The presentwork evaluated fibrinolytic and proteolytic secretion abilities of 35 Agaricomycetes isolates fromthe Paranaense rainforest (Misiones, Argentina). We detected proteolytic activity in 40% of thestrains while nine strains showed fibrinolytic activity. Schizophyllum commune LBM 026,Schizophyllum commune LBM 223, and Hornodermoporus martius LBM 224 exhibited the highestlevels of fibrinolytic activity. Fibrin zymography from S. commune LBM 026 and LBM 223 showed an enzyme of 27.5 kDa, while H. martius LBM 224 presented an enzyme of 29 kDa. The evaluation of the enzymatic stability of culture supernatant of these strains revealed that the fibrinolytic activity was highly stable over a wide temperature and pH range. Long-term stability of fibrinolytic activity at physiological conditions evidenced that the strains had a halflife of at least 72 h. Fibrinolytic enzymes produced by S. commune LBM 026 and LBM 223 were inhibited in the presence of EDTA indicating that they are metalloproteases. This work reveals the potential of S. commune LBM 026, S. commune LBM 223, and H. martius LBM 224 as an unconventional source of thrombolytic agents.