LINE-1 Retrotransposon activation in non-tumorigenic mammary epithelial cells exposed to pesticides. A link to why is breast cancer on the rise?.

MIRET NOELIA V; ZAPPIA C. DANIEL; CAROLINA A PONTILLO; ZARATE LORENA; LEGUIZAMON FLAVIA; LASAGNA MARIANELA; COCCA CLAUDIA; MONCZOR FEDERICO; ANDREA RANDI

Ciudad Autónoma de Buenos Aires

Congreso; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

SAIC

Pesticides exposure is linked to the rise in breast cancer incidence. Long interspersed nuclear element-1 (LINE-1) is inhibited in somatic tissues but its reactivation was implicated in mammary tumorigenesis. The pesticides hexachlorobenzene (HCB) and chlorpyrifos (CPF), both ligands of the aryl hydrocarbon receptor (AhR), increase LINE-1 activation in breast cancer cells MDA-MB-231, through AhR/c-Src and TGF-β1/Smad3 signaling. Our aim was to evaluate if LINE-1 can be activated by pesticide exposure in non-tumorigenic mammary epithelial cells NMuMG. We used environmentally relevant doses of CPF (0.05-50 µM) and HCB (0.005-5 µM). LINE-1 mRNA levels were examined by RT-qPCR, finding an increment after 48 h of exposure with CPF (0.05-5 µM, p