Expression of long interspersed nuclear element-1 (LINE-1) is reactivated during breast cancer initiation and progression. Strong ligands of aryl hydrocarbon receptor (AhR) activate LINE-1 through the transforming growth factor-β1 (TGF-β1)/Smad pathway. S

LASAGNA MARIANELA; CLARA VENTURA; HIELPOS MARIA SOLEDAD; MARDIROSIAN, MARIANA; MIRET NOELIA V; ANDREA RANDI; MARIEL NÚÑEZ; CLAUDIA COCCA

Ciudad Autónoma de Buenos Aires

Congreso; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

SAIC

Chlorpyrifos (CPF) is a pesticide whose effects on breast cancer risk are not clear. Our aim was to analyze if CPF promotes migration and the pathways involved in MCF-7 and MDA-MB-231 cell lines. Migration was evaluated by 2D (wound healing and Boyden Chamber assays) and 3D assays (multicellular spheroids). c-Src, AKT, P38 and GSK3-β were studied by Western Blot. In MCF-7 cells, 0.05 µM CPF induced the migration after 48 h (p