G-PROTEIN-COUPLED ESTROGEN RECEPTOR GPER MEDIATES IMIDACLOPRID-INDUCED MAMMARY EPITHELIAL CELL MIGRATION

LEGUIZAMÓN M. AGUSTINA; BUJÁN SOL; CAROLINA A PONTILLO; CHIAPPINI FLORENCIA; LASAGNA MARIANELA; CLAUDIA COCCA; RANDI ANDREA; MIRET NOELIA

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI & FAIC SAFIS 2022; 2022

SAIC SAI & FAIC SAFIS

Imidacloprid (IMI) is a neonicotinoid insecticide widely used in agriculture worldwide. It is detected in fruits and vegetables, as well as in human serum and urine. Although neonicotinoids are considered to be of low toxicity to mammals compared to other insecticides, increasing studies show the potential risk to humans of IMI exposure. IMI is an endocrine disruptor that enhances aromatase activity and estradiol secretion in breast cancer cells. G protein-coupled estrogenreceptor (GPER) is activated by estradiol and estrogenic compounds, promoting cell migration, metalloprotease (MMP) activity, and epithelial-mesenchymal transition processes in breast cancer.Due to these antecedents, we hypothesize that IMI exposure produces alterations in the mammary gland that favor tumorigenesis. In order to evaluate the potential impact of IMI on mammary epithelial cells, we worked with the NMuMG cell line exposed to environmentally relevant doses of IMI (0.01-10 μM) or vehicle (DMSO) for 24 hours. First, the possible cytotoxic effect of IMI was studied (MTT assay), finding no effect on NMuMG cell viability. However, when examining the levels of the proliferation marker PCNA (Western blot, WB), we found a reduction in their levels at 1 μM (40% p