CHLORPYRIFOS INDUCES LUNG METASTASIS AND MODULATION OF CANCER STEM CELL MARKERS IN IN VIVO MODEL

LASAGNA MARIANELA; ZAPPIA C. DANIEL; MARDIROSIAN MARIANA NOELIA; MIRET NOELIA; ANDREA RANDI; DAHIR LARA SOLEDAD; NUÑEZ MARIEL; COCCA CLAUDIA

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI & FAIC SAFIS 2022; 2022

SAIC SAI & FAIC SAFIS

Breast cancer was the most frequently diagnosed malignant tumor in Argentina in 2020. In recent decades, exposure to different environmental pollutants, many of them endocrine disruptors (Eds) such as Chlorpyrifos (CPF), have been associated with an increased risk of breast cancer development and progression. To evaluate whether CPF was able to promote metastasis in vivo, we used an experimental model N: NIH athymic female mice in which tumors derived from the MDA-MB-231 cell line were xenotransplanted. They were randomly divided into 3 groups: control (C), CPF 0.001 mg/ kg/day (CPF 0.001) or CPF 0.1 mg/kg/day (CPF 0.1) and treated for 22 days. The tumor doubling time was significantly shorter in CPF exposed animals (8.1 days for CPF 0.001 and 6.3 days for CPF 0.1) than C (9.4 days). Tumors from animals exposed to both doses of CPF showed greater cellular pleomorphism, anisokaryosis, apoptotic bodies and hyperchromatic nuclei, and appeared to have a lower degree of differentiation. We found a larger lung metastasis area in CPF 0.001 compared to C (p