BECAS
LASAGNA Marianela
artículos
Título:
Chronic exposure to low concentrations of chlorpyrifos affects normal cyclicity and histology of the uterus in female rats
Autor/es:
RAMOS NIETO, MARÍA ROSA; LASAGNA, MARIANELA; CAO, GABRIEL; ÁLVAREZ, GLORIA; SANTAMARIA, CLARISA; RODRIGUEZ GIRAULT, MARÍA EUGENIA; BOURGUIGNON, NADIA; DI GIORGIO, NOELIA; VENTURA, CLARA; MARDIROSIAN, MARIANA; RODRIGUEZ, HORACIO; LUX-LLANTOS, VICTORIA; COCCA, CLAUDIA; NÚÑEZ, MARIEL
Revista:
FOOD AND CHEMICAL TOXICOLOGY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2021 vol. 156
ISSN:
0278-6915
Resumen:
Chlorpyrifos (CPF), the most used insecticide in Argentina, can act as an endocrine disruptor at low doses. We previously demonstrated that chronic exposure to CPF induces hormonal imbalance in vivo. The aim of this work was to study the effects of low concentrations of CPF (0.01 and 1 mg/kg/day) on the reproductive system of virgin adult rats. In the ovary, we studied the effects of CPF on steroidogenesis by determining steroid hormone content by RIA and CYP11 and CYP19 enzyme expression by qRT-PCR. The estrous cycle was evaluated by microscopic observation of vaginal smear, as well as by changes in uterine histology. In endometrium, we determined the fractal dimension and expression of PCNA, ERα and PR by IHC. Our results showed that chronic exposure to CPF affects ovarian steroid synthesis, causing alterations in the normal cyclicity of animals. In addition, CPF induced proliferative changes in the uterus, suggesting that it could affect reproduction or act as a risk factor in the development of uterine proliferative pathologies.