Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd.

FERNÁNDEZ ME; GOSZCZYNSKI DE; LIRÓN JP,; VILLEGAS-CASTAGNASSO EE; CARINO MH; RIPOLI MV; ROGBERG-MUÑOZ A; POSIK DM,; PERAL-GARCÍA P; GIOVAMBATTISTA G

GENETICS AND MOLECULAR BIOLOGY

SOC BRASIL GENETICA

Año: 2013 vol. 36 p. 185 - 191

1415-4757

During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal geneticidentification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) havebeen increasingly used for this purpose. An efficient SNP identification system requires a marker set with enoughpower to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals.In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with thatprovided by STRs. Thirty-six closely related Angus cattle were genotyped for 18 STRs and 116 SNPs. CumulativeSNPs exclusion power values (Q) for paternity and sample matching probability (MP) yielded values greater than0.9998 and 4.32E-42, respectively. Generally 2-3 SNPs per STR were needed to obtain an equivalent Q value. TheMP showed that 24 SNPs were equivalent to the ISAG (International Society for Animal Genetics) minimal recommendedset of 12 STRs (MP ~ 10-11). These results provide valuable genetic data that support the consensus SNPpanel for bovine genetic identification developed by the Parentage Recording Working Group of ICAR (InternationalCommittee for Animal Recording).