Telomere Length Analysis in Mody Type Diabetes Patients

MILLÁN ANDREA LILIANA; TROBO SOFÍA IRENE; DE DIOS ALEJANDRO; PÉREZ MARÍA SILVIA; CERRATO GARCÍA MARTINA; FRECHTEL GUSTAVO; LÓPEZ ARIEL PABLO

Mendoza

Simposio; II International Symposium On Translational Medicine XIII Annual Meeting of Investigation at The Faculty of Medical Sciences.; 2019

Faculty of Medical Sciences; Faculty of Exact and Natural Sciences at Universidad Nacional de Cuyo; Faculty of Pharmacology and Biochemistry at Universidad de Buenos Aires and the Faculty of Medicine at Albert-Ludwigs-Universität Freiburg, Germany.

Introduction and Objectives:MODY is a heterogeneous and non-insulin-dependent form of monogenic diabetes, being MODY2 and MODY3 types the most common, characterized by moderate hyperglycemia. Telomere shortening is involved in the replicative capacity of a cell, so it is considered marker of cellular aging.Since the chronic hyperglycemia present in MODY patients can influence the telomere length (TL) as well as in type 2 diabetes (T2D), and the TL status is not known in such patients, the aim was to evaluate and compare the TL between individuals genetically diagnosed as MODY2 or MODY3 with non-diabetic controls and T2D patients.Methods:We analyzed 33 patients with genetic diagnosis of MODY2, 12 with MODY3 and 48 non-diabetic individuals, matched by sex and age. In other hand, we selected 18 MODY patients from the cohort and 18 T2D age-matched with recent clinical diagnose. Absolute telomere length (aTL) was performed by absolute quantification by qPCR. The relation between the kpb of telomeric sequences and the number of copies of the single copy gene RPLPO (ratio T/S) was measured. Statistical analysis was carried in SPSS with a significance level of 0.05.Results:Whole sample (MODY and controls) showed a correlation between aTL and age (p=0.002). We found that both MODY patients had a shorter aTL compared to the controls (p=0.023). However, we did not find significant differences in aTL between both types of MODY, but in comparison with T2D patients, the aTL was significantly more shorter (p