Surface chemistry modification of silica nanoparticles alters the activation of monocytes

SARACENO, MARTIN; MITAROTONDA, ROMINA; TODONE, MARCOS; GIORGI, EXEQUIEL; MALCHIODI, EMILIO L.; DESIMONE, MARTI´N F.; DE MARZI, MAURICIO C.

Therapeutic Delivery

Future Medicine Ltd.

Año: 2021 vol. 12 p. 443 - 459

2041-5990

Aim: Nanoparticles (NPs) interaction with immune system is a growing topic of study. Materials and methods: Bare and amine grafted silica NPs effects on monocytes/macrophages cells were analyzed by flow cytometry, MTT test and LIVE/DEAD®viability/cytotoxicity assay. Results: Bare silica NPs inhibited proliferation and induced monocyte/macrophages activation (increasing CD40/CD80 expression besides pro-inflammatory cytokines and nitrite secretion). Furthermore, silica NPs increased cell membrane damage and reduced the number of living cells. In contrast, amine grafted silica NPs did not alter these parameters. Conclusion: Cell activation properties of bare silica NPs could be hindered after grafting with amine moieties. This strategy is useful to tune the immune system stimulation by NPs or to design NPs suitable to transport therapeutic molecules. © 2021 Newlands Press.