N-TERMINAL TRANSIALIDASE EXPRESSED IN RECOMBINANT BCG CONFER PROTECTION IN MURINE MODEL OF Trypanosoma cruzi INFECTION

LIHUE GONZALEZ; KAREN LEAL; ESTEFANÍA PROCHETTO; LUZ RODELES; LUZ PEVERENGO; GIULIANA LUPI; CAROLINA ROLDAN; SIBELE BORSUK; ODDIR DELLAGOSTI; IVÁN MARCIPAR; IVÁN BONTEMPI

Ciudad Autónoma de Buenos Aires

Congreso; Reunion Conjunta de Sociedades de Biociencias; 2017

SAP

In a previous work, we have expressed different antigens of Trypanosomacruzi (T. cruzi) in recombinant bacilo calmette y Guerin(BCG) and assessed the constructions as vaccine candidates in amurine infection model. The more promising candidate correspondedto the amino terminal fraction of the trans-sialidase (nTS). In thiswork we compared the immunogenicity of nTS when it is expressedin two BCG plasmids, which differ in their expression promoter inBCG; the nTS-Pus977 and the nTS-Pus2000. Additionally, we assessedthe protection of these constructions in a highly virulent infectionof T. cruzi.BALB/c mice (n=6) were immunized every 30 days twice with nTSPus2000,nTS-Pus977, or BCG and PBS as controls groups. 30 daysafter the last immunization, significant delayed-type hypersensitivity(DTH) reactivity of nTS constructions were obtained compared tocontrols groups (p