PARTICIPACIÓN DEL TEJIDO ADIPOSO HUMANO EN LA REGULACIÓN DE LA PROGRESIÓN TUMORAL EN EL CÁNCER DE RIÑÓN

BRUNA F; ROMEO LR; CAMPOVERDE- ARBOCCO F.; CONTADOR D; LOPEZ LAUR JD; GOMEZ SE; SANTIANO FE; SASSO CV; ZYLA LE; LOPEZ-FONTANA CM; CARON RW; PISTONE-CREDYT V

San Luis

Congreso; sociedad de Biología de Cuyo; 2017

SOCIEDAD ARGENTINA DE INVESTIGACIONES CLINICAS

We have recently demonstrated that human adipose tissue of tumor kidney [hRAT], showed leptin and versican overexpressed compare to adipose tissue of normal kidney [hRAN]. Meanwhile, adiponectin and perilipin were significantly increased in hRAN. Conditioned media (CMs) of hRAN significantly increased proliferation of tumor [ACHN] and non-tumor [NK-2] renal epithelial cells. Now, we evaluated the expression of receptors (R): adiponectin -1, -2 (AdipoR1/R2) and leptin (ObR) in [hRAN vs hRAT] and [ACHN vs HK-2]. Additionally, CD44 expression (homing cell adhesion molecule) was assessed in cells. Human renal adipose tissues were obtained from patients with renal cell carcinoma [hRAT, n=6] and kidney donors [hRAN, n=8]. The CMs of hRAN and hRAT were collected 24h post incubation and [ACHN vs HK-2] were treated with CMs by 24 h. Protein expression was evaluated in tissue by IHQ, and WB in cells. Statistical differences among experimental conditions were evaluated by one-way ANOVA or t test, with Tukey´s post hoc tests. We found AdipoR1 increased and ObR decreased in hATN vs. hATT (p