HUMAN RENAL ADIPOSE TISSUE FROM NORMAL AND TUMORAL KIDNEY REGULATES THE BEHAVIOR OF RENAL EPITHELIAL CELLS

CAMPOVERDE- ARBOCCO F.; BRUNA F; ROMEO LR; CONTADOR D; GOMEZ SE; SASSO CV; SANTIANO FE; ZYLA LE; LOPEZ-FONTANA CM; CARON RW; PISTONE-CREDYT V

San Luis

Congreso; sociedad de Biología de Cuyo; 2017

Sociedad Biología

Tumor stroma is one of the emerging topics of interest in cancer research. In the present work, we evaluated the effects of Conditioned Media (CMs) of human adipose tissue explants from normal (hRAN) and tumor (hRAT) kidney on proliferation of tumor (786-O, ACHN) and non tumor (NK-2) renal epithelial cells. We also evaluated: 1) leptin, adiponectin and versican mRNA expression in hRAN and hRAT; 2) adiponectin, ADAMTS1 and perilipin expression in hRAN- and hRAT-CMs; and 3) adiponectin and perilipin expression in hRAN- and hRAT.Human renal adipose tissues were obtained from patients with renal cell carcinoma (hRAT, n=6) and kidney donors (hRAN, n=8). The CMs from hRAN and hRAT were collected after 24h of incubation. 786-O, ACHN and NK-2 cells were grown and incubated with CMs for 24 and 48h. Proliferation was quantified by MTT; mRNA expression was evaluated by real-time PCR, protein expression by WB and immunohistochemistry. Statistical differences between experimental conditions were evaluated by one-way ANOVA or t test. Tukey´s post hoc tests were performed.We found a higher RNA expression of leptin (a major adipokine with tumorigenic effects) and versican (a proteoglycan that is essential in epithelial-stromal interaction and intracellular signaling) in hRAT vs. hRAN explants (p