Expressed Sequences Tags de Tecidos Reprodutivos de Machos de Anastrepha fraterculus (Tephritidae)

PASCUAL, AMALIA NELCI TERESITA; MALAGÓ JR, WILSON; SILVA, FLÁVIO HENRIQUE; DE BRITO, REINALDO ALVES

Viña del Mar. Chile

Congreso; XIV Congreso Latinoamericano de Genética (ALAG), VIII Congreso de la Asociación Latinoamericana de Mutagénesis, Carcinogénesis y Teratogénesis Ambiental (ALAMCTA), XLIII Congreso de la Sociedad de Genética de Chile (SOCHIGEN), XXXIX Congreso de la Socieda; 2010

Asociación Latinoamericana de Genética

RESUMEN 504 EXPRESSED SEQUENCES TAGS DE TECIDOS REPRODUTIVOS DE MACHOS DE Anastrepha fraterculus (TEPHRITIDAE) (ESTs from males reproductive tissues of Anastrepha fruit flies) Pascual, A.N.T., Malagó Jr,W., Silva,F.H. y Brito R.O.A. Depto Genética y Evolución, CCBS, UFSCar, Rod. Washington Luís-km 235 São Carlos,SP, Brasil. nelcitpascual@yahoo.com.br Expressed sequences tags (ESTs) from genomic libraries are a useful tool for insect evolutionary studies. Anastrepha fraterculus is a complex of recently diverged fruit flies species well represented in Brazil. Even though most of the sexual selection pressure is thought to be on males, there are no reproductive tissues libraries described for any other Tephritidae species so far. The aim of this work was to build a partial genomic cDNA library of A. fraterculus males to generate ESTs that might contribute with identification of candidate genes involved in differentiation and speciation. Partial cDNA library was constructed with Cloneminer Kit (Invitrogen) from reproductive system dissected from 105 males of A.fraterculus collected from different localities in Brazil. Clones were processed and submitted for analyses in the dCAS pipeline: 2713 useful ESTs resulted in 1307 contigs (1032 singletons and 275 overlapping sequences). Length of contigs ranged from 100 to 2667bp, variation of GC% of 12.68-54.98%. PolyA tails were found in 530 contigs (base position 68 to 2638bp). ESTs were categorized using GO terms (Gene Ontology Consortium, 2000) and 643 (49,19%) corresponded to hits of Drosophila, three Tephritidae genera and other known Diptera species. Our preliminary description from male transcriptome unbeknown until now have revealed several genes that could facilitate future speciation and other genome insect studies.