CHEMICAL AND DOSE-DEPENDENT EFFECT OF TITANIUM AND SILICA NANOPARTICLES OVER MACROPHAGES

GIORGI, EXEQUIEL DAVID; GENOVÉS, SOFIA; DIAZ, SOFÍA; DIAZ, MARIA EUGENIA; DESIMONE, MARTIN; DE MARZI, MAURICIO CESAR

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2022

Nanoparticles (NPs) are particles below 100 nm of diameter and they can generate different biological effects depending on their chemical composition, size, shape and morphology. Our objective is to compare the effect of silica and titanium dioxide NPs over a mouse macrophage cell line (RAW 264.7), evaluating cell metabolic activity and nitrites expression. Titanium dioxide NPs were provided by Evonik (AEROXIDE TiO2 P 25). As colloidal silica model we used LUDOX®. Size and shape of the NPs were analyzed by TEM and microimages were processed by ImageJ software. NPs were also analyzed by FTIR and DLS. Mouse macrophage cells (RAW 264.7) were cultured with different concentration (25-500 µg/mL) of NPs for 24-96 h. Cell supernatants were analyzed by Griess reaction to measure nitric oxide expression in macrophages supernatants culture. Cell metabolic activity was determined by MTT assay. A significant decrease on cell metabolic activity in presence of LUDOX® was observed, showing a major effect at higher NPs concentrations (500, 250 and 100 µg/mL) and longer times (P